Genome Editing: Tools to Support CRISPR/Cas9 Applications

Genome editing is enabled by the development of tools to make precise, targeted changes to the genome of living cells. Recently a new tool based on a bacterial CRISPR-associated protein-9 nuclease (Cas9) from Streptococcus pyogenes has generated considerable excitement. CRISPR-based genome editing workflows vary considerably depending on experimental design. NEB reagents support multiple workflows enabling researchers flexibility in their genome editing strategy. In this presentation we will introduce NEB’s RNA synthesis products, novel assembly methods for generation of sgRNA templates and homologous repair templates, recombinant Cas9 protein, and reagents for downstream mutation analysis.