Crop Innovation Using Sequence Based Genotyping

Next Generation Sequencing has revolutionized the life sciences including crop improvement. Over the past decade KeyGene has leveraged the power of NGS in its Accelerated Molecular Breeding platform to develop a portfolio of proprietary Sequence Based Genotyping approaches suiting the needs of today's molecular plant breeders in terms of quality, cost, flexibility and turn-around times.
Sequence-Based Genotyping (SBG) technology enables simultaneous discovery and genotyping of novel polymorphisms in any germplasm using restriction enzyme-mediated complexity reduction approaches; at present many forms of SBG including GBS, RAD-Seq and related methods are widely used for crop genotyping. KeyGene® SNPSelect is complementary to SBG as it provides a fully customizable, highly flexible NGS-based targeted genotyping solution for preselected polymorphisms at unparalleled scale and competitive cost per data-point. Our whole genome re-sequencing approaches in combination with fast variant detection enable high resolution analysis of germplasm including detection of rare variants.

Based on these methods, KeyGene offers tailor-made solutions to all plant breeder's genotyping needs.

The SBG and KeyGene® SNPSelect technologies are protected by patents and patent applications owned by KeyGene N.V..