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Genome Technology: Sequencing

P0068
Comprehensive Total RNA Seq to Capture Both the mRNA and Small RNA Information in a Single Assay
Beena PS, AgriGenome Labs Pvt Ltd; Bipin Balan, AgriGenome Labs Pvt Ltd; Raja Durai, AgriGenome Labs Pvt Ltd; Tintu Joy, AgriGenome Labs Pvt Ltd; Saju Michael, AgriGenome Labs Pvt Ltd; Dhinoth Kumar Bangarusamy, AgriGenome Labs Pvt Ltd; V B Reddy Lachagari, AgriGenome Labs Pvt Ltd

P0069
A Novel Method for Complete Environmental Microbial Profiling
Alvaro G. Hernandez, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign; Chris L. Wright, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign; Mark A. Mikel, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign; Mark Band, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign

P0070
Innovations in de novo Whole Genome, Transcriptome and Metagenomic Sequencing
Chris L. Wright, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign; Mark Band, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign; Mark A. Mikel, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign; Alvaro G. Hernandez, Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign

P0071
16S V5 V6 Metagenomics Kit Offers Enhanced Bacterial Identification in Plant Associated Samples
Bradley Hehli, Bioo Scientific; Jessica Cogdill, Bioo Scientific; Carrie Firmani, Bioo Scientific; Kevin Allen, Bioo Scientific; Masoud Toloue, Bioo Scientific

P0072
De novo Sequencing of the Vitis vinifera cv. Flame Seedless Genome using Single Pass Library Preparation from Swift Biosciences
Sara Goodwin, Cold Spring Harbor Laboratory; Lance Cadle-Davidson, USDA-ARS Grape Genetics Research Unit; Michael S. Campbell, Cold Spring Harbor Laboritory; Xia Xu, ARS-Grape Genetic Research Unit; Robert Wappel, Cold Spring Harbor Laboratory; Craig A. Ledbetter, Crop Diseases, Pests and Genetics Research Unit, USDA-ARS; Rachael Naegele, USDA-ARS Crop Diseases, Pests and Genetics Research,; W. Richard McCombie, Cold Spring Harbor Laboratory; Doreen Ware, Cold Spring Harbor Laboratory

P0073
Efficient High-Throughput SNP Discovery and Genotyping Using LGC’s Normalised Genotyping-By-Sequencing (nGBS) Method
Joris Parmentier, LGC Genomics Ltd; Knut Mohr, LGC Genomics

P0074
Haplotype Reconstruction Using Linked-Reads
Claudia Catalanotti, 10x Genomics Inc.; Stephane Boutet, 10x Genomics Inc.; Sarah Garcia, 10x Genomics Inc.; Haynes Heaton, 10X Genomics Inc.; David Jaffe, 10x Genomics Inc.; Sofia Kyriazopoulou-Panagiotopoulou, 10x Genomics Inc.; Vijay Kumar, 10x Genomics Inc.; Patrick Marks, 10x Genomics Inc.; Michael Schnall-Levin, 10x Genomics Inc.; Preyas Shah, 10x Genomics Inc.; Matt Sooknah, 10x Genomics Inc.; Grace Zheng, 10x Genomics Inc.; Deanna Church, 10x Genomics Inc.; Andrew Wei Xu, 10x Genomics Inc.; Neil Weisenfeld, 10x Genomics Inc.

P0075
Micro-RNA Expression Profiles and Gene Target Prediction in Atlantic Salmon Infected with Caligus rogercresseyi
Valentina Valenzuela-Muñoz, Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research. University of Concepción, Chile.; Gustavo Núñez-Acuña, Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research. University of Concepción, Chile.; Diego Valenzuela-Miranda, Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research. University of Concepción, Chile.; Ana T. Gonçalves, Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research. University of Concepción, Chile.; Camille Détrée, Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research. University of Concepción, Chile.; Sebastian Boltaña, University of Concepción, Chile.; Cristian Gallardo, Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research. University of Concepción, Chile.

P0076
Targeted Resequencing in Peanuts Using the Fluidigm Access Array
Roshan Kulkarni, Texas Tech University; Ratan Chopra, USDA- ARS; Jennifer Chagoya, Texas A&M AgriLife Research; Steven Hoffman, Fluidigm; Phlip Bocock, Fluidigm; Petar Grozdanov, TTU HSC - core facility; Charles E. Simpson, Texas A & M Agrilife research; Mike Baring, Texas A & M Agrilife research; Naveen Puppala, New Mexico State University; Andrew Hillhouse, Department of Veterinary Pathobiology, Texas A&M University College of Veterinary Medicine; Mark D. Burow, Texas A&M AgriLife Research, Lubbock, and Department of Plant and Soil Science, Texas Tech University

P0077
Sequencing-Based Mapping of Resistance to Leaf Spots and the Prominent Main Stem of Tifrunner by using TILLING Lines and BSA-Seq Approach in Groundnut (Arachis hypogaea L.)
Annapurna Chitikineni, ICRISAT; Hui Wang, University of Georgia, Department of Plant Pathology; Aamir W Khan, ICRISAT; Gaurav Agarwal, University of Georgia; Manish K Pandey, ICRISAT; Peggy Ozias-Akins, University of Georgia; Corley Holbrook, University of Georgia; Rajeev K Varshney, ICRISAT; Baozhu Guo, USDA-ARS, Crop Protection and Management Research Unit

P0078
A Rapid Method for End-to-End Assembly of Complex Genomes, Pathogen Discovery, and Metagenomic Deconvolution
Ivan Liachko, University of Washington - Department of Genome Sciences; Shawn Sullivan, Phase Genomics, Inc; Jay Shendure, University of Washington - Department of Genome Sciences; Maitreya Dunham, University of Washington

P0079
Variant Identification Workflow for Chromosome Scale Assembly
Marta Matvienko, Qiagen; Alex Kozik, UC Davis; Jens Nielsen, Qiagen; Arne Materna, Qiagen

P0080
Optimized Sample Prep Methods for Long-Range Genomics and de novo Assembly
Susana Wang Jett, 10x Genomics, Inc.; Nikka Keivanfar, 10x Genomics, Inc.; Rabeea Abbas, 10x Genomics, Inc.; Jill Herschleb, 10x Genomics Inc.

P0082
Full-Length cDNA Sequencing on the PacBio Sequel Platform
Ting Hon, Pacific Biosciences; Elizabeth Tseng, Pacific Biosciences; Aparna Vedula, Pacific Biosciences; Tyson A. Clark, Pacific Biosciences

P0083
De Novo Genome Sequencing and Mapping of the Crown-of-Thorns Sea Star, Acanthaster planci
Ryo Koyanagi, Okinawa Institute of Science and Technology; Kenneth Baughman, Okinawa Institute of Science and Technology; Nori Satoh, Okinawa Institute of Science and Technology

P0084
Implementation of a High Throughput AgriSeqTM Workflow for GBS
Angela Burrell, Thermo Fisher Scientific; Michelle Swimley, Thermo Fisher Scientific; Chris Willis, Thermo Fisher Scientific; Adam F. Allred, Thermo Fisher Scientific; Prasad Siddavatam, Thermo Fisher Scientific; Daniel Kephart, Thermo Fisher Scientific

P0085
Ion Ampliseq Targeted Sequencing Panel for Determination of Bovine Parentage
Prasad Siddavatam, Thermo Fisher Scientific; Angela Burrell, Thermo Fisher Scientific; Adam F. Allred, Thermo Fisher Scientific

P0086
Synthetic Long Read Sequencing with Optical Mapping Produces High Quality de novo Genomes
Alan F. Scott, Johns Hopkins University Sch of Med; David W. Mohr, Johns Hopkins University Sch of Med

P0087
Genome-Wide Identification of Histone Acetylation (H4K12ac) and Methylation (H3K9me2) Marks in Switchgrass (Panicum virgatum L.)
Vasudevan Ayyappan, Delaware State University; Sripathi Venkatswara Rao, Alabama A&M University; Malay C. Saha, Noble Research Institute; Jyothi Thimmapuram, Purdue University; Ketaki Bhide, Bioinformatics Core, Purdue University; Venu (Kal) Kalavacharla, Delaware State University

P0088
Detection of Active Transposable Elements in Arabidopsis thaliana using Oxford Nanopore Sequencing Technology
Emilie Debladis, University of Perpignan; Marie-Christine Carpentier, University of Perpignan; Christel Llauro-Berger, University of Perpignan; Marie Mirouze, Institut of Research for Development (IRD); Olivier Panaud, University of Perpignan

P1255
Whole Genome Profiling Based Physical Map of Field Pea
Krishna Kishore Gali, University of Saskatchewan; Bunyamin Taran, University of Saskatchewan; Mohammed-Amin Madoui, CEA - Genoscope; Edwin van der Vossen, Keygene; Karine Labadie, CEA - Genoscope; V B Reddy Lachagari, AgriGenome Labs Pvt Ltd; Hélène Berges, Centre National de Ressources Génomiques Végétales (CNRGV); Judith Burstin, INRA, UMR1347 Agroécologie; Tom Warkentin, University of Saskatchewan

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