Development and Mapping New Microsatellite Markers in Lentil

Lentil (Lens culinaris Medik.) is an important crop worldwide for its nutritional values and agronomic benefits. The genome size of lentil is 4063Mb/C across 2n=2x=14 chromosomes. Most of the linkage maps of lentil were constructed using random and dominant markers such as RAPD, ISSR and AFLP markers. However, microsatellites or simple sequence repeat (SSR) markers have been widely used in many crop species due to their abundance, high degree of polymorphism, locus specificity, reproducibility and co-dominance heredity. Although microsatellite based markers are available, they are limited in number in lentil. Recently, we developed around 200 SSR markers from four microsatellites enriched libraries and integrated them into three different mapping RIL populations namely CDC Redberry x ILL 7502, PI 320937 x Eston, ILL 8006-BM x CDC .